Full-length RecE and RecT from Rac prophage mediate highly efficient linear–linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression ...

The cloning of long DNA segments, especially those containing large gene clusters, is of particular importance to synthetic and chemical biology efforts for engineering organisms. While cloning has ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Integrated DNA Technologies (IDT) has announced the availability of its miRCat™ small RNA cloning system. The kit-based resources make the creation of small RNA (including miRNAs, piRNAs and ...

Nereus Gunther is a molecular biologist at the Agricultural Research Service within the US Department of Agriculture (Washington D.C., USA), where part of his research focuses on Campylobacter and its ...