本研究通过头对头比较Vircell、Certest和Altona三种HDV RNA qRT-PCR检测试剂盒,揭示了其在定性诊断上高度一致(κ=0.988-1.000),但存在显著的定量偏倚(Vircell和Certest分别高估0.33和0.24 log10IU/mL)。同时,研究证实热休克(thermal shock)前处理虽能提升低病毒载量样本的 ...
Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...
Polymerase chain reaction (PCR) tests have become routine because of the COVID-19 pandemic, with more than a million PCR tests being carried out daily in the United States alone. What are now commonly ...