In typical complementary DNA microarray experiments, two fluorescently labeled RNAs are hybridized to an array of cDNA probes on a glass slide, and their relative gene expression intensities, ratios ...

Most public-founded large-scale sequencing projects that use cDNAs are primarily using cDNA libraries which are not enriched for full-length cDNAs. Consequently, only a fraction of the resulting ESTs ...

The main steps involved and limitations of microarray analysis will be encountered. Two stages can be distinguished in microarray experiments: a pre-microarray experiment phase (tissue handling) and ...

Within the International Life Sciences Institute Committee on Genomics, a working group was formed to focus on the application of microarray technology to preclinical assessments of drug-induced ...

To identify differentially expressed genes and scan candidate molecular markers in nasopharyngeal carcinoma (NPC). We constructed four subtracted cDNA libraries using suppression subtractive ...

In this issue we take a close look at DNA microarrays, the current amore of biological and biomedical researchers. There's little reason to doubt that the infatuation will continue, at least for ...

Current microarray analysis uses 'chips' containing either 25-residue oligonucleotides synthesized by photolithography or cDNAs placed by robotic spotting. In the April Nature Biotechnology, Hughes et ...

You are oncologists specializing in breast cancer and will be conducting a microarray analysis on one of two newly diagnosed breast cancer patients, Mrs. Jones and Mrs. Brown. You will be adding a ...

In each type of cell, like a muscle cell or a skin cell, different genes are expressed (turned on) or silenced (turned off). If the cells that are turned on mutate, they could—depending on what role ...

A 32,000 cDNA microarray was used to evaluate 78 human colon cancer specimens, and these results were correlated with survival. Molecular classifiers were produced to predict outcome. These data ...