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生物通
10 年
流式细胞技术新工具:PCR+流式分析
Abate提出了PCR激活细胞分拣方法:PACS(PCR-activated cell sorting)。每个液滴都包含有一个单细胞,PCR试剂,靶向靶标序列的引物,以及一个荧光TaqMan探针。成功扩增细胞中的DNA,就能得到一个荧光信号,然后通过激光微流控电路进行检测,由此来分拣。 生物通报道 ...
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